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KMID : 1094720110160020366
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 2 p.366 ~ p.373
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Gene cloning and expression of a 3-ketovalidoxylamine C-N-lyase from Flavobacterium saccharophilum IFO 13984
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Kim Jong-Gyun
Joo Jeong-Chan
Kim Soo-Kwang
Yoo Young-Je
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Abstract
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In this study, we report the first functional cloning and heterogeneous expression of 3-ketovalidoxylamine C-N lyase (E.C. 4.3.3.1) from Flavobacterium saccharophilum IFO 13984. This gene is 1,098 bp in length and encodes a peptide of 366 amino acids. The recombinant C-N lyase was successfully overexpressed in E. coli, and its functional activity, degradation of 3-ketovalidoxylamine A, was confirmed by HPLC analysis. The sequence and phylogenetic analysis showed that the C-N lyase has no similarity with other amine lyases (E.C. 4.3.3) but has similarity with the conserved domain present in SusD and RagB. Thus, the C-N lyase may have a similar binding domain for sugar moieties with SusD/RagB. This genetic information may lead to improvements in C-N lyase function for industrial applications.
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KEYWORD
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3-ketovalidoxylamine C-N lyase, Flavobacterium saccharophilum, gene cloning, expression
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